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Thattathin Marayathu Voot 2018 Tamil Movie watch online.[Construction of a quantitative plant-specific random-primed transcriptome library based on de novo assembly and sequencing].
As a high throughput and efficient means of expression profiling, the cDNA sequencing is widely used in plant functional genomics and proteomics. In this paper, using the Illumina high-throughput sequencing platform, we constructed a cDNA library of Sinapis alba L. cv ‘White gold’, a typical temperate mustard variety, with the aim of establishing a quantitative plant-specific random-primed transcriptome library. Based on the de novo assembly and sequencing result, the complexity of the Sinapis alba L. cv ‘White gold’ transcriptome library was evaluated. The cDNA sequencing and assembly were performed on the Illumina HiSeq 2000 platform. The transcriptome sequencing data were analyzed by SOAPdenovo software, the genome assembly result was obtained by using the Trinity software, and the sequencing quality was evaluated by the SOAPnuke software. A total of 233 616 201 clean reads with a length of 150-500 bp were obtained from 11 829 594 raw reads, and 63 158 transcripts were obtained by de novo assembly. Through the simulation experiment and the clustering analysis, the length and the number of reads had little effect on the transcriptome complexity, but they were the main factors affecting the assembly efficiency. The length distribution of the Sinapis alba L. cv ‘White gold’ transcripts was unevenly dispersed. The average length of the transcripts ranged from 559 bp to 13 936 bp. Based on the functional annotation, out of the 63 158 transcripts, 26 912 (43.22%) transcripts were functionally annotated in gene ontology, while the remaining transcripts did not match any genes in the public database. Compared with the public database, the genome assembly result was more accurate. In addition, 68 405 (28.06%) homologous sequences were found in the transcriptome dataset of Sinapis
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