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Click here to view the Discussion page for this entry.Purification and characterization of two cytochrome c oxidases in mitochondria from Neurospora crassa.
Two cytochrome c oxidase activities have been purified from Neurospora crassa (baker’s yeast). Activities 1 and 2 were isolated using a molybdate-sensitive (activity 1) or a molybdate-stabilized (activity 2) procedure on mitochondria. Their partial amino acid sequence is different from that of the cytochrome c oxidase reported previously in Neurospora crassa. The electron transfer kinetics of the two preparations was studied. The activation of the molybdate-stabilized form 2 by the addition of a superoxide dismutase showed a biphasic behavior with apparent Ki values in the range of 0.2 to 0.4 microM. No difference in kinetic properties between the two preparations was found. Oxidation of cytochromes b and c were found to be essential for the inhibition of activity 2 by the diazonium radical (NIO2+) in a concentration-dependent manner. The inhibition was a rapid process showing that the diazonium radical is able to bind to the enzyme.The AEC2017 Honorary Awards Night held in Beijing on July 26th has been successfully held. On the gala night, we were honored to accept the Award from Chinese Minister of Culture Ma Xulun. China is the host of the world’s biggest cultural event, so the Chinese Minister of Culture Ma Xulun congratulates and welcomes us.
DZCMC International is bringing a new face to Chinese wind power for the first time. A new generation of wind power has arrived. Five internationally renowned wind power plants, the leading developers and operators, will be gradually ramped up in the coming three to five years.
Hydropower in China remains the largest source of renewable energy; in 2017 it contributed 59.3% of new renewables energy. Hydropower accounted for 3.65% of the global total in 2017. After more than 40 years of development, China’s hydroelectric capacity rose to 1,272,529 GWh by the end of 2017.
China’s coal consumption in 2017 was lower than in the previous two years, but at a high rate of growth
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